Ho ea ka tsela e kopanyang ea amino acid le tsoekere, peptide ea tsoekere e ka aroloa ka mekhahlelo e mene: O glycosylation, C a N glycosylation, phoka saccharification le GPI (glycophosphatidlyinositol) khokahano.
1. N-glycosylation glycopeptides e entsoe ka N-acetamide glucose qetellong ea ho fokotsa ketane ea glycan (Glc-Nac) e kopantsoeng le athomo ea N ho sehlopha sa amide sa ketane e ka thōko ea Asn e 'ngoe ea ketane ea peptide, le Asn. e khonang ho hokahanya ketane ea glycan e tlameha ho behoa ho AsN-X-Ser / Thr (X! = P) ka motif e entsoeng ke masala.Tsoekere ke N-acetylglucosamine.
N-glycosylation e fetotsoeng sebopeho sa glycopeptide
2. Sebopeho sa O-glycosylation se bonolo ho feta N-glycosylation.Glycopeptide ena ka kakaretso e khuts'oane ho feta glycan, empa e na le mefuta e mengata ho feta N-glycosylation.Ser le Thr ka kakaretso li ka ba glycosylated ka har'a ketane ea peptide.Ho feta moo, ho na le li-glycopeptides tse khabisitsoeng ka tyrosine, hydroxyl, le hydroxyproline glycosylation.Sebaka sa sehokelo ke athomo ea oksijene ea hydroxyl e lehlakoreng la ketane ea masala.Tsoekere e hokahantsoeng ke galactose kapa N-acetylgalactosamine (Gal&GalNAc) kapa glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), joalo-joalo.
O-glycosylation e fetola sebopeho
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
N-acetylglucosamine (GlcNAc) glycosylation e le 'ngoe e kopanya liprotheine tsa O-GlcNAc le athomo ea hydroxyl ea oksijene ea serine kapa masalla a threonine a protheine.O-GlcNA glycosylation ke O-GlcNAc monosaccharide mokhabiso ntle le glycan katoloso;Joalo ka peptide phosphorylation, O-GlcNAc glycosylation ea glycopeptides le eona ke ts'ebetso e matla ea ho khabisa liprotheine.Mokhabiso o sa tloaelehang oa O-GlcNAc o ka baka mafu a fapaneng a kang lefu la tsoekere, mafu a pelo, lihlahala, lefu la Alzheimer's joalo-joalo.
Lintlha tsa glycosylation tsa glycopeptides
Mehaho ea mantlha ea polypeptide le liketane tsa tsoekere li hokahane le liketane tsa protheine ka li-covalent bond, 'me libaka tse hokahanyang liketane tsa tsoekere li bitsoa libaka tsa glycosylation.Kaha ha ho na template ea ho latela biosynthesis ea liketane tsa tsoekere ea glycopeptide, liketane tse fapaneng tsa tsoekere li tla kenngoa sebakeng se tšoanang sa glycosylation, se lebisang ho seo ho thoeng ke microscopic inhomogeneity.
Glycosylation ea glycopeptides
1. Phello ea glycopeptide glycosylation kalafong e sebetsang hantle ea liprotheine tsa kalafo.
Tabeng ea liprotheine tsa kalafo-phekolo, glycosylation e boetse e ama halofo ea bophelo le sepheo sa lithethefatsi tsa protheine ka har'a vivo.
2. Soluble glycopeptide glycosylation le liprotheine
Boithuto bo bontšitse hore liketane tsa tsoekere tse holim'a liprotheine li ka ntlafatsa ho qhibiliha ha limolek'hule tsa liprotheine
3. Glycopeptide glycosylation le protheine immunogenicity
Ka lehlakoreng le leng, liketane tsa tsoekere tse holim'a liprotheine li ka hlahisa likarabo tse khethehileng tsa 'mele oa ho itšireletsa mafung.Ka lehlakoreng le leng, liketane tsa tsoekere li ka koahela libaka tse itseng holim'a protheine le ho fokotsa matla a eona a ho itšireletsa mafung
4. Glycopeptide glycosylation e eketsang botsitso ba protheine
Glycosylation e ka eketsa botsitso ba liprotheine maemong a fapaneng a denaturation (joalo ka li-denaturants, mocheso, joalo-joalo) le ho qoba ho bokellana ha liprotheine.Ka nako e ts'oanang, liketane tsa tsoekere tse holim'a liprotheine li ka boela tsa koahela lintlha tse ling tsa ho senyeha ha proteolytic ea limolek'hule tsa protheine, ka hona, ho eketsa khanyetso ea liprotheine ho liprotheine.
5. Glycopeptide glycosylation e amang ts'ebetso ea tlhaho ea limolek'hule tsa protheine
Ho fetola protheine glycosylation ho ka boela ha thusa limolek'hule tsa protheine ho theha mesebetsi e mecha ea likokoana-hloko
Nako ea poso: Aug-03-2023