Phosphorylation e ama likarolo tsohle tsa bophelo ba lisele, 'me protheine kinases e ama likarolo tsohle tsa mesebetsi ea puisano ea intracellular ka ho laola litsela tsa lipontšo le mekhoa ea lisele.Leha ho le joalo, phosphorylation e sa tloaelehang e boetse e baka mafu a mangata;haholo-holo, protheine kinases le phosphatase e fetotsoeng e ka baka mafu a mangata, 'me chefo e ngata ea tlhaho le likokoana-hloko le tsona li na le phello ka ho fetola boemo ba phosphorylation ea liprotheine tsa intracellular.
Phosphorylation ea serine (Ser), threonine (Thr), le tyrosine (Tyr) ke ts'ebetso ea phetoho ea protheine e fetohang.Ba kenya letsoho taolong ea mesebetsi e mengata ea cellular, joalo ka pontšo ea li-receptor, mokhatlo oa liprotheine le karohano, ho kenya tšebetsong kapa ho thibela tšebetso ea protheine, esita le ho phela ha lisele.Phosphates e qosoa hampe (litefiso tse peli tse mpe ka sehlopha sa phosphate).Ka hona, tlatsetso ea bona e fetola thepa ea protheine, eo hangata e leng phetoho ea conformational, e lebisang phetohong ea sebopeho sa protheine.Ha sehlopha sa phosphate se tlosoa, sebopeho sa protheine se tla khutlela boemong ba sona ba pele.Haeba liprotheine tse peli tsa conformational li bonts'a mesebetsi e fapaneng, phosphorylation e ka sebetsa joalo ka phetoho ea limolek'hule bakeng sa protheine ho laola ts'ebetso ea eona.
Li-hormone tse ngata li laola mosebetsi oa li-enzyme tse khethehileng ka ho eketsa boemo ba phosphorylation ea masala a serine (Ser) kapa threonine (Thr), 'me tyrosine (Tyr) phosphorylation e ka hlahisoa ke lintlha tsa ho hōla (tse kang insulin).Lihlopha tsa phosphate tsa li-amino acid tsena li ka tlosoa kapele.Kahoo, Ser, Thr, le Tyr li sebetsa joalo ka li-switches tsa limolek'hule taolong ea mesebetsi ea cellular joalo ka ho ata ha hlahala.
Li-peptide tsa maiketsetso li bapala karolo ea bohlokoa haholo thutong ea li-protein kinase substrates le tšebelisano.Leha ho le joalo, ho na le lintlha tse ling tse sitisang kapa tse fokotsang ho ikamahanya le maemo ha theknoloji ea phosphopeptide synthesis, joalo ka ho se khone ho fihlela othomathike e felletseng ea mokhahlelo o tiileng le khaello ea khokahano e bonolo le sethala se tloaelehileng sa tlhahlobo.
Sethala se thehiloeng ho peptide synthesis le phosphorylation modification technology se hlola mefokolo ena ha se ntse se ntlafatsa ts'ebetso ea motsoako le scalability, 'me sethala se loketse hantle bakeng sa boithuto ba protheine kinase substrates, antigen, limolek'hule tse tlamang, le li-inhibitors.
Nako ea poso: May-31-2023