Liquid chromatograph ke chromatograph e bohlale e shebaneng le basebelisi, e nang le ts'ebetso ea mantlha ea HPLC e tloaelehileng, 'me e atolosa mesebetsi e bohlale haholoanyane.E ka kopana hantle le litlhoko tse fapaneng tsa ts'ebeliso ea basebelisi, e le hore basebelisi ba ka e sebelisa habonolo le ho fumana lintlha tse nepahetseng tsa tlhahlobo.
Ntlha ea pele, molao-motheo:
Molao-motheo oa ts'ebetso e phahameng ea chromatography ea mokelikeli o ipapisitse le chromatography ea khale ho latela mohopolo oa meteorological color harmonic, kholomo e sebelisoa ka mokhoa o ikhethileng ka likaroloana tse nyane, sephetho ke hore katleho ea kholomo e phahame haholo ho feta ea khale ea khale. liquid color harmonic, e ka boela ea ba le mochine o nang le kutloelo-bohloko ka mor'a tšebeliso ea kholomo.E nolofalletsa ho lemoha ho tsoelang pele ha mohlahlobi ea tsoang.
Chromatograph ke ts'ebeliso ea motsoako oa likarolo mohatong o tsitsitseng le phallo ea mobile phase, kabo kapa adsorption ea liphapang tsa ts'ebetso ea tšebelisano ea lik'hemik'hale, e le hore likarolo tse motsamaong o lekanyelitsoeng oa mekhahlelo e 'meli li kenngoe khafetsa ke matla a kaholimo ho fihlela karohano. .Li-chromatograph tsa ts'ebetso e phahameng li sebelisoa haholo tlhahlobong ea lijo, tlhahlobo ea tikoloho, saense ea bophelo, laboratori ea bongaka le tlhahlobo ea inorganic."Ka kakaretso, liperesente tse 80 ho isa ho tse 85 tsa lintho tse phelang li ka hlahlojoa ke HPLC."
Ii.Tšebeliso ea Lisebelisoa:
Chromatograph ke sehlahisoa sa chromatograph se sebelisoang hangata.Ke sesebediswa se arolang motswako pele ebe se sekaseka le ho supa motswako ka ho sebedisa phapano ya karolelano ya kabo mahareng a metsi a tiileng kapa a sa qhibidihang.Likarolong tse tsebahalang tsa manyolo, hoo e ka bang 80% e ka aroloa le ho hlahlojoa ke chromatography ea mokelikeli e sebetsang hantle, 'me hobane maemo a mokhoa ona a bonolo, ha a senye sampole, ka hona e loketse haholo metsoako ea manyolo le lintho tse phelang tse nang le ts'ebetso e matla. boemo bo belang bo phahameng, ho thatafalloa ho ts'oara le ho senya, le botsitso bo fokolang ba mocheso.
Liquid chromatograph e sebelisoa haholo ho biochemistry, tlhahlobo ea lijo, lipatlisiso tsa meriana, tlhahlobo ea tikoloho, tlhahlobo ea inorganic le likarolo tse ling.E ka laola liparamente tsohle le ts'ebetso ea chromatograph, ea kenya ts'ebetso ea ho hlophisa, 'me ea itlhahloba ka bo eona lisampole tsa tatellano.Pontšo ea nako ea 'nete ea chromatogram, kopanyo le liphetho tsa tlhahlobo ea tlaleho, ho taka curve e tloaelehileng, joalo-joalo.
Nako ea poso: May-26-2023