Li-antigen tsa protheine tse kopanetsoeng hangata li na le li-epitopes tse fapaneng tse fapaneng, tse ling tsa tsona ke li-epitopes tse latellanang 'me tse ling ke li-epitopes tsa sebopeho.Li-antibodies tsa Polyclonal tse fumanoeng ka ho enta liphoofolo ka li-antigen tse entsoeng ke denatured ke motsoako oa li-antibodies tse ikhethileng ho li-epitopes ka bomong, 'me li ka sebelisoa ka kakaretso ho lemoha meaho ea tlhaho kapa liprotheine tse shebiloeng.Molemo o mong oa ho sebelisa liprotheine tsa denatured e le li-immunogens ke hore liprotheine tse senyehileng li atisa ho ba immunogenic 'me li ka susumetsa karabelo e matla ea ho itšireletsa mafung ho liphoofolo.
Mokhoa oa ho bua oa Escherichia coli hangata o khethoa bakeng sa merero ea antigen hobane ke tsamaiso e theko e boima ka ho fetisisa ho latela nako le chelete.E le ho ntlafatsa monyetla oa polelo ea protheine e lebisitsoeng le boiketlo ba tlhoekiso, ka linako tse ling ho hlahisoa karoloana e nyane feela ea protheine e lebisitsoeng, joalo ka sebaka se itseng.
Sebopeho sa likarolo tse tharo tsa liprotheine
Sebaka se Khethehileng sa Protheine
Haeba sepheo sa ho lokisoa ha li-antibody e le feela bakeng sa ho lemoha wb, ho na le moruo ebile ho potlakile ho sebelisa peptide e nyenyane ea maiketsetso e le antigen, empa ho na le kotsi ea ho fokola ha 'mele oa ho itšireletsa mafung kapa ho se ts'oanelehe ka lebaka la khetho e sa lokelang ea karolo ea peptide.Kaha ho lokisoa ha li-antibody ho hloka nako e telele, likarolo tse peli kapa tse tharo tse fapaneng tsa peptide hangata li khethoa ho lokisa li-antibodies ho sebelisa polypeptide antigen ho netefatsa sekhahla sa katleho ea teko.
Bohloeki ba antigen ea polypeptide bakeng sa ente bo hlokeha ho feta 80%.Le hoja bohloeki bo phahameng bo ka fumana li-antibodies ka mokhoa o hlakileng haholoanyane, ha e le hantle, liphoofolo li lula li hlahisa palo e kholo ea li-antibodies tse sa tobang, kahoo li pata melemo ea bohloeki ba antigen.
Ho feta moo, ho lokisoa ha li-antibodies tse tsoang ho li-peptide tse nyane li tlameha ho hokahanngoa le antigen e tsamaisang e loketseng ho matlafatsa boitšireletso ba eona.Lijari tse peli tse tloaelehileng tsa antigenic ke KLH le BSA.
Nako ea poso: Mar-23-2023