Ho na le mefuta e mengata ea li-peptide tsa transmembrane, 'me lihlopha tsa tsona li ipapisitse le thepa ea' mele le ea lik'hemik'hale, mehloli, mekhoa ea ho ja, le lits'ebetso tsa biomedical.Ho ea ka thepa ea bona ea 'mele le ea lik'hemik'hale, li-peptide tse kenang lera li ka aroloa ka mefuta e meraro: cationic, amphiphilic le hydrophobic.Li-peptide tse phunyeletsang tsa cationic le amphiphilic li etsa 85%, ha li-peptide tse phunyeletsang tsa hydrophobic li etsa 15% feela.
1. Lera la cationic le phunyeletsang peptide
Li-peptide tsa Cationic transmembrane li entsoe ka li-peptide tse khutšoane tse ruileng ka arginine, lysine, le histidine, joalo ka TAT, Penetratin, Polyarginine, P22N, DPV3 le DPV6.Har'a tsona, arginine e na le guanidine, e ka kopanyang hydrogen le lihlopha tse mpe tsa phosphoric acid ka lera la sele le ho hokahanya li-peptide tsa transmembrane ka har'a lera tlas'a boemo ba PH ea 'mele.Liphuputso tsa oligarginine (ho tloha ho 3 R ho ea ho 12 R) li bontšitse hore bokhoni ba ho kenella ha membrane bo ne bo finyelloa feela ha palo ea arginine e le tlase ho 8, 'me bokhoni ba ho kenella ha membrane butle-butle bo ile ba eketseha ka keketseho ea palo ea arginine.Lysine, le hoja e le cationic joaloka arginine, ha e na guanidine, kahoo ha e le mong, katleho ea eona ea ho kenella ha lera ha e phahame haholo.Futaki et al.(2001) e fumane hore phello e ntle ea ho kenella ha membrane e ka finyelloa feela ha cationic cell membrane e phunyeletsang peptide e nang le bonyane li-amino acid tse 8 tse qosoang hantle.Le hoja masala a amino acid a qosoang hantle a le bohlokoa hore li-peptide tse kenang li kene ka har'a lera, li-amino acid tse ling li bohlokoa ka ho lekana, joalo ka ha W14 e fetoha F, ho kenella ha Penetratin hoa lahleha.
Sehlopha se ikhethileng sa cationic transmembrane peptides ke nuclear localization sequences (NLSs), e nang le li-peptide tse khutšoane tse ruileng ka arginine, lysine le proline 'me li ka isoa khubung ka nyutlelie ea pore complex.Li-NLS li ka boela tsa aroloa ka mokhoa o le mong le oa ho thaepa habeli, o nang le sehlopha se le seng le tse peli tsa li-amino acid tsa motheo, ka ho latellana.Mohlala, PKKKRKV ho tsoa ho simian virus 40(SV40) ke NLS e tlanya e le 'ngoe, ha protheine ea nyutlelie e le NLS e tlanya habeli.KRPAATKKAGQAKKKL ke tatelano e khuts'oane e ka bapalang karolo ho membrane transmembrane.Hobane li-NLS tse ngata li na le linomoro tsa tefiso tse ka tlase ho 8, li-NLS ha se li-peptide tsa transmembrane tse sebetsang hantle, empa li ka ba li-peptide tse sebetsang hantle tsa transmembrane ha li hokahane hantle le tatellano ea hydrophobic peptide ho etsa li-amphiphilic transmembrane peptide.
2. Amphiphilic transmembrane peptide
Li-amphiphilic transmembrane peptides li na le libaka tsa hydrophilic le hydrophobic, tse ka aroloa ka li-amphiphilic tsa mantlha, tsa bobeli tsa α-helical amphiphilic, β-folding amphiphilic le prolin-enriched amphiphilic.
Mofuta oa mantlha oa li-amphiphilic wear membrane peptides ka mekhahlelo e 'meli, sehlopha se nang le li-NLS tse hokahaneng ka tatellano ea hydrophobic peptide, joalo ka MPG (GLAFLGFLGAAGSTMGAWSQPKKKRKV) le Pep - 1 (KETWWETWWTEWSQPKKRKV), Ka bobeli li ipapisitse le lets'oao la nyutlelie leo PKK4phoc0RKV. domain of MPG e amana le tatelano ea fusion ea HIV glycoprotein 41 (GALFLGFLGAAGSTMG A), 'me sebaka sa hydrophobic sa Pep-1 se amana le sehlopha se ruileng sa tryptophan se nang le membrane e phahameng (KETWWET WWTEW).Leha ho le joalo, libaka tsa hydrophobic tsa bobeli li hokahane le lets'oao la nyutlelie la PKKKRKV ka WSQP.Sehlopha se seng sa li-peptide tsa mantlha tsa amphiphilic transmembrane li ne li arohane le liprotheine tsa tlhaho, tse kang pVEC, ARF(1-22) le BPrPr(1-28).
Li-peptide tsa bobeli tsa α-helical amphiphilic transmembrane li tlamella lera ka α-helices, 'me masala a bona a hydrophilic le hydrophobic amino acid a fumaneha libakeng tse fapaneng tsa sebopeho sa helical, joalo ka MAP (KLALKLALK ALKAALKLA).Bakeng sa mofuta oa amphiphilic wear membrane ea mofuta oa beta peptide, bokhoni ba eona ba ho etsa letlapa la beta bo bohlokoa ho matla a eona a ho kenella ka har'a membrane, joalo ka VT5 (DPKGDPKGVTVTVTVTVTGKGDPKPD) ts'ebetsong ea ho etsa lipatlisiso ka bokhoni ba ho kenella ha lesela, o sebelisa mofuta oa D. - li-analogues tsa phetoho ea amino acid ha li khone ho theha sekhechana se phuthoang sa beta, bokhoni ba ho kenella ha lera bo bobe haholo.Ka proline-enriched amphiphilic transmembrane peptides, polyproline II (PPII) e thehoa habonolo metsing a hloekileng ha proline e ruisitsoe haholo mohahong oa polypeptide.PPII ke helix e ka letsohong le letšehali e nang le masalla a 3.0 amino acid ka nako e le 'ngoe, ho fapana le sebopeho se tloaelehileng sa letsoho le letona la alpha-helix se nang le masalla a 3.6 amino acid ka lehlakoreng le leng.Proline-enriched amphiphilic transmembrane peptides e kenyeletsa bovine antimicrobial peptide 7(Bac7), synthetic polypeptide (PPR)n(n e ka ba 3, 4, 5 le 6), joalo-joalo.
3. Hydrophobic membrane e kenang peptide
Li-peptide tsa Hydrophobic transmembrane li na le mesaletsa feela ea amino acid e seng polar, e nang le tefiso e ka tlase ho 20% ea tefiso eohle ea tatellano ea amino acid, kapa e na le likarolo tsa hydrophobic kapa lihlopha tsa lik'hemik'hale tse bohlokoa bakeng sa transmembrane.Leha li-peptide tsena tsa cellular transmembrane hangata li hlokomolohuoa, li teng, joalo ka fibroblast growth factor (K-FGF) le fibroblast growth factor 12(F-GF12) from Kaposi's sarcoma.
Nako ea poso: Mar-19-2023