Mokhoa oa ho ntša
Lilemong tsa bo-1950 le bo-1960, linaha tse ngata lefatšeng, ho kenyeletsoa China, haholo-holo li ile tsa ntša li-peptide ho tsoa lithong tsa liphoofolo.Ka mohlala, ente ea thymosin e lokisoa ka ho hlaba namane e sa tsoa tsoaloa, ho tlosa thymus ea eona, ebe ho sebelisoa theknoloji ea karohano ea oscillating ho arola peptide ho namane thymus.Thymosin ena e sebelisoa haholo ho laola le ho ntlafatsa ts'ebetso ea 'mele ea ho itšireletsa mafung ho batho.
Li-peptide tsa tlhaho tsa bioactive li ajoa haholo.Ho na le li-peptide tse ngata tsa bioactive liphoofolong, limela le linthong tse phelang metsing ka tlhaho, tse bapalang mesebetsi e fapaneng ea 'mele le ho boloka mesebetsi e tloaelehileng ea bophelo.Li-peptide tsena tsa tlhaho tsa bioactive li kenyelletsa metabolite ea bobeli ea lintho tse phelang joalo ka lithibela-mafu le lihomone, hammoho le li-peptide tse sebetsang tse teng lits'ebetsong tse fapaneng tsa lisele.
Hajoale, li-peptide tse ngata tsa bioactive li qheletsoe ka thoko ho batho, liphoofolo, limela, likokoana-hloko le lintho tse phelang metsing.Leha ho le joalo, li-peptide tsa bioactive ka kakaretso li fumaneha ka bongata bo fokolang linthong tse phelang, 'me mekhoa ea hajoale ea ho arola le ho hloekisa li-peptide tsa bioactive ho tsoa ho lintho tsa tlhaho ha e ea phethahala, ka theko e phahameng le bioactivity e tlase.
Mekhoa e tloaelehileng e sebelisoang bakeng sa ho ntša peptide le ho arohana e kenyelletsa ho hlahisa letsoai, ultrafiltration, gel filtration, isoelectric point precipitation, ion exchange chromatography, affinity chromatography, adsorption chromatography, gel electrophoresis, joalo-joalo.
Mokhoa oa acid-base
Acid le alkali hydrolysis li sebelisoa haholo litsing tsa liteko, empa ha li sebelisoe hangata ts'ebetsong ea tlhahiso.Ts'ebetsong ea alkaline hydrolysis ea liprotheine, boholo ba li-amino acid tse kang serine le threonine lia senyeha, ho etsahala racemization, 'me palo e kholo ea limatlafatsi e lahlehile.Ka hona, mokhoa ona ha o sebelisoe ka seoelo tlhahiso.Acid hydrolysis ea liprotheine ha e bake racemization ea amino acid, hydrolysis e potlakile mme karabelo e felile.Leha ho le joalo, bofokoli ba eona ke theknoloji e rarahaneng, taolo e thata le tšilafalo e tebileng ea tikoloho.Kabo ea boima ba limolek'hule ea li-peptide ha e lekane ebile ha e tsitsa, 'me ho thata ho tseba hore na mesebetsi ea bona ea mmele e sebetsa joang.
Enzymatic hydrolysis
Li-peptide tse ngata tsa bioactive li fumaneha liketane tse telele tsa liprotheine sebakeng se sa sebetseng.Ha hydrolyzed ke protease e itseng, peptide ea bona e sebetsang e lokolloa tatellanong ea amino ea protheine.Ho ntšoa ha Enzymatic ea bioactive peptide ho tloha liphoofolong, limela le linthong tse phelang metsing e 'nile ea e-ba taba ea lipatlisiso lilemong tse mashome tsa morao tjena.
Enzymatic hydrolysis ea bioactive peptide ke khetho ea li-protease tse nepahetseng, tse sebelisang liprotheine joalo ka substrates le hydrolyzing proteins ho fumana palo e kholo ea li-peptide tsa bioactive tse nang le mesebetsi e fapaneng ea fisioloji.Ts'ebetsong ea tlhahiso, mocheso, boleng ba PH, khatello ea enzyme, mahloriso a substrate le lintlha tse ling li amana haufi-ufi le phello ea enzymatic hydrolysis ea li-peptide tse nyane, 'me senotlolo ke khetho ea enzyme.Ka lebaka la li-enzyme tse fapaneng tse sebelisetsoang enzymatic hydrolysis, khetho le tlhahiso ea li-enzyme, le mehloli e fapaneng ea liprotheine, li-peptide tse hlahisoang li fapana haholo ka boima, kabo ea boima ba limolek'hule, le sebopeho sa amino acid.Hangata motho o khetha li-protease tsa liphoofolo, tse kang pepsin le trypsin, le li-protease tsa limela, tse kang bromelain le papain.Ka nts'etsopele ea mahlale le mahlale le ntlafatso e tsoelang pele ea theknoloji ea enzyme ea baeloji, ho tla sibolloa li-enzyme tse ngata le ho sebelisoa.Enzymatic hydrolysis e sebelisitsoe haholo ho lokiseng li-peptide tsa bioactive ka lebaka la theknoloji ea eona e holileng le matsete a tlase.
Nako ea poso: May-30-2023