Lipatlisisong tsa baeloji, li-polypeptides tse nang le tatellano e telele hangata li sebelisoa.Bakeng sa li-peptide tse nang le li-amino acid tse fetang 60 ka tatellano, polelo ea liphatsa tsa lefutso le SDS-PAGE hangata li sebelisoa ho li fumana.Leha ho le joalo, mokhoa ona o nka nako e telele mme phello ea ho qetela ea karohano ea lihlahisoa ha e ntle.
Mathata le tharollo bakeng sa motsoako o molelele oa peptide
Ka har'a motsoako oa li-peptide tse telele, re lula re tobana le bothata, ke hore, tšitiso ea steric ea karabelo ea condensation e eketseha ka keketseho ea tatellano ea motsoako, 'me nako ea karabelo e hloka ho lokisoa ho etsa hore karabelo e phethehe.Leha ho le joalo, ha nako e ntse e feta, ho na le litla-morao tse ngata tse hlahisoang, 'me karolo ea peptide e reretsoeng e thehoa.Mesaletsa e joalo - liketane tsa peptide tse haellang ke litšila tse ka sehloohong tse hlahisoang ka nako e telele ea peptide synthesis.Ka hona, ts'ebetsong ea peptide e telele, bothata ba bohlokoa boo re tlamehang ho bo hlola ke ho hlahloba maemo a boemo bo holimo ba karabelo le mekhoa ea karabelo, e le ho etsa hore karabelo ea amino acid e be e felletseng le e felletseng.Ho phaella moo, fokotsa nako ea ho arabela, hobane ha nako e telele ea ho itšoara ka nako e telele, ho feta ho sa laoleheng ho sa laoleheng ka lehlakoreng, ho thata haholoanyane ho hlahisa lihlahisoa.Ka hona, lintlha tse tharo tse latelang li akaretsoa:
Microwave synthesis e ka sebelisoa: Bakeng sa li-amino acid tse ling tse kopaneng le ts'ebetso ea ho kopanya tse seng bonolo ho kopanngoa, motsoako oa microwave o ka sebelisoa.Mokhoa ona o na le liphello tse tsotehang, 'me o fokotsa haholo nako ea ho itšoara,' me o fokotsa ho thehoa ha lihlahisoa tse peli tsa bohlokoa.
Ho ka sebelisoa mokhoa oa ho kopanya likaroloana: Ha li-peptide tse ling li le thata ho etsoa ka mekhoa e tloaelehileng ea ho kopanya 'me ho se bonolo ho hloekisoa, re ka amohela condensation eohle ea li-amino acid tse' maloa karolong e itseng ea peptide ho ketane ea peptide ka kakaretso.Mokhoa ona o ka boela oa rarolla mathata a mangata ka ho kopanya.
Acylhydrazide synthesis e ka sebelisoa: acylhydrazide synthesis ea li-peptide ke mokhoa o tiileng-phase synthesis ea N-terminal Cys peptide le C-terminal polypeptide hydrazide chemical selective reaction pakeng tsa ho thehoa ha li-amide bonds ho finyella mokhoa oa ho kopanya peptide.Ho ipapisitsoe le boemo ba Cys ketane ea peptide, mokhoa ona o arola ketane eohle ea peptide ka tatellano e mengata ebe oa e kopanya ka ho latellana.Qetellong, peptide e shebiloeng e fumanoa ka karabelo ea mokelikeli-phase ea condensation.Mokhoa ona ha o fokotse haholo nako ea ho kopanya peptide e telele, empa hape o eketsa haholo bohloeki ba sehlahisoa sa ho qetela.
Ho hloekisa peptide e telele
Bokhabane ba li-peptide tse telele bo lebisa ho likarolo tse rarahaneng tsa li-peptide tse tala.Ka hona, hape ke phephetso ho hloekisa li-peptide tse telele ka HPLC.letoto la li-amyloid tsa ts'ebetso ea tlhoekiso ea polypeptide, e nkang boiphihlelo bo bongata mme e sebelisoa ka katleho tlhoekisong ea peptide e telele.Ka ho amohela lisebelisoa tse ncha, ho kopanya mekhoa e mengata ea ho hloekisa, ho arohana khafetsa le mekhoa e meng ea phihlelo, tekanyo ea katleho ea ho hloekisa peptide e telele e ile ea ntlafala haholo.
Nako ea poso: Apr-18-2023