Mokhoa o mocha oa Thymus o entsoe ka 28 amino acid synthetic polypeptide immune regulator.Mokhoa oa Thymus ke mofuta oa litlhare tse ncha tsa uct, tse sebelisoang hangata ho bakuli ba hlahala, li ka matlafatsang sesole sa 'mele.
Mokhoa oa ts'ebetso
Mokhoa o nepahetseng oa thymalfasin kalafo ea lefu la sebete le sa foleng la B ha o e-so ithutoe ka botlalo, empa liteko tse fapaneng li bonts'itse hore thymalfasin e na le karabelo ea bohlokoa ea 'mele ea ho itšireletsa mafung.Haholo-holo e finyelloa ka ho ntlafatsa ts'ebetso ea lisele tsa T, 'me e feta har'a linaha tse kenyelletsang tlhahiso ea lisele tsa T, karohano le ntlafatso, le apoptosis.Ka ho eketsa palo e felletseng ea lymphoid, 'molai oa tlhaho oa intrahepatic, CD3 le CD4 lisele, thymalfasin e ka bapala karolo ea immunomodulatory.Pleural fasin e ka khothaletsa kholo le karohano ea lisele tsa DC, ka hona, e khothaletsa kholo le karohano ea lisele tsa T.Thymalfasin e ka kenya tšebetsong mitogen kapa antigen, ea ntlafatsa tlhahiso ea mefuta e fapaneng ea li-cytokine joalo ka interferon human interleukin IL2 le IL3, ea ntlafatsa polelo ea interleukin-2 receptor, le ho etsa hore lisele tsa CD4T li arohane ho THLS, tse nang le litlamorao tse kholo tsa antiviral.Ka ho eketsa karolo ea P cell, e ka khothaletsa tlhahiso ea li-lymphocyte ka har'a vivo, ea fetola karolo ea P cell subsets le U cell differentiation, hammoho le tlhahiso ea li-antibodies, e susumetsa tlhahiso ea li-lymphokine tse ling tsa antiviral le macrophages, ho ntlafatsa ho metsa. sebetsa, le ho ntlafatsa ts'ebetso ea sesole sa 'mele ho bakuli ba mofetše.E ka boela ea tlosa libaktheria, libaktheria le likokoana-hloko 'meleng.Buka ea mokhoa oa Thymus bakeng sa tsamaiso ea methapo le endothelium e potolohileng, e phahama ho fetola karolo ea phetiso ea hippocampal neuron synaptic.Thymus mokhoa o mocha oa phetiso ea synaptic le tsamaiso ea methapo, e ka sebelisoa ho thibela bakuli ba mofetše ba bakoang ke kalafo ea mofetše joalo ka chemotherapy le neurotoxicity.Pleuraxin e na le litlamorao tsa taolo ea liphatsa tsa lefutso, e ka susumetsa liphatsa tsa lefutso tsa kinase, ho eketsa liphatsa tsa lefutso tsa cytokine, liphatsa tsa lefutso tsa ho itšireletsa mafung.The up-regulation of thymalfasin on IL-like receptor genes e fana ka maikutlo a hore thymalfasin e susumetsa lisele tse hlahisang antigen.Ho feta moo, phello ea thymalfasin kholong ea lisele tsa 'mele ea ho itšireletsa mafung e bonahatsoa ke polelo e ntseng e eketseha ea lintlha tsa kholo ea lisele tsa stem le liphatsa tsa lefutso tsa protheine ea hematopoietic.
Tatelano e ncha ea thymectomy
Mokhoa oa ho lokisetsa
1. Mokhoa oa ho ntša lik'hemik'hale tsa biochemical Litokisetso tsa Thymosin (motsoako oa li-peptide) li fumanoa ka har'a lisele tsa liphoofolo, kahoo mosebetsi o fapane haholo.Hobane e se e na le protheine ea liphoofolo, ho na le monyetla o moholo oa matšoao a ho kula.Mokhoa o fumanoeng ka biochemical oa thymus ho tsoa ho embryonic thymus e ncha, e nang le chai e tlase haholo empa e sebetsa hantle haholo.Ha ho nahanoa ka lisebelisoa tse fokolang tse tala, theko e phahameng, chai e tlase, le mokhoa o rarahaneng oa ho reka., mathata a maholo a tlhahiso, tebello ea tsoelo-pele ha e na tšepo.
2. Tšebeliso ea lik'hemik'hale thymusfasin e fumanehang khoebong e hlahisoa haholo-holo e le SPSS, e hlahisoang le ho hloekisoa ka mekhoa e nepahetseng.Ho ipapisitsoe le resin, Fmoc e ne e sebelisetsoa ho theha karolo e tiileng, 'me mefuta e 28 ea li-amino acid e entsoe ho latela thymalfasin.N-terminal amino acid e ne e le acetylated le acetic anhydride, 'me TFA e ne e sebelisoa e le setsi sa bohlokoa sa ho senya bakeng sa ho senya ho fumana lihlahisoa tse thata.Sehlahisoa se sa tsoakoang se ile sa aroloa ke HPLC ho fumana sehlahisoa se secha sa mokhoa oa thymus, se ileng sa hloekisoa le ho feta ho fumana thepa e tala ea mokhoa oa thymus.
Ho na le mefuta e meraro ea libaktheria tse entsoeng ka liphatsa tsa lefutso
1) Liphatsa tsa lefutso tse ngata li entsoe ka har'a moamoheli ho fumana liphatsa tsa lefutso tse lebisitsoeng, 'me qetellong li hlahisoa ka mokhoa oa' mele oa kenyelletso.Ho tlalehiloe hore mofuta oa "thymmethod" oa mofuta oa likhoele tse ngata o thehiloe ka mekhoa e sa reroang ea annealing le PCR mme ea phetoleloa ho Escherichia coli.(2) Mofuta oa protheine ea fusion o hlahisitsoeng o ile oa kopanngoa hape mecheng ea litaba, 'me mecha ea litaba e ile ea fetoloa hore e be moamoheli ho aha libaktheria tse entsoeng ka liphatsa tsa lefutso.Mohlala, ho ipapisitsoe le mofuta oa peptide oa plasmid, polelo ea plasmid e ile ea hahuoa, ea fetoloa Pichia pastoris ka electroexcitation, ebe protheine ea fusion e hlahisoa ke methanol, ebe mokhoa o mocha oa thymus o hahiloe ka tšilo ea enzyme le mekhoa e meng. .
Nako ea poso: Sep-08-2023