Li-peptide tse fetotsoeng ka methylation, tse tsejoang hape e le methylation-recognized peptide, ke mekhabiso ea liprotheine tsa morao-rao (PTMs) 'me li bapala karolo ea bohlokoa ea taolo hoo e batlang e le mesebetsi eohle ea bophelo liseleng.Liprotheine li hlahisoa ke methyltransferase ho fetisetsa lihlopha tsa hydroxyl ho masalla a itseng a amino acid bakeng sa tlamahano e kopanetsoeng.Methylation ke mokhoa o fetohang oa phetoho o hlahisoang ke demethylases.
Liphuputso li bontšitse hore li-amino acid tse tloaelehileng tsa methylated/demethylated hangata ke lysine (Lys) le arginine (Arg).Liphuputso li fumane hore histone lysine methylation e na le mesebetsi e sa tšoaneng ea likokoana-hloko tse kang ho boloka lisele tsa stem le karohano, X chromosome inactivation, regulation ea transcript le karabo ea tšenyo ea DNA.", hangata e ama chromatin condensation mme e hatella polelo ea lefutso."Histone arginine methylation e bapala karolo ea bohlokoa taolong ea ho ngoloa ha liphatsa tsa lefutso mme e ka ama lits'ebetso tse fapaneng tsa 'mele ka liseleng, ho kenyeletsoa tokiso ea DNA, phetisetso ea mats'oao, nts'etsopele ea lisele le lefu la kankere.Ka hona, Guopeptide Biology e ntlafalitse ka ho khetheha theknoloji ea li-peptide tse khabisitsoeng tsa methyl, tse fetotsoeng ke bo-ramahlale kamora phetolelo ea protheine (PTMS) ho thusa lipatlisisong.
Phetoho ea methylation (Me1, Me2, Me3)
Fmoc-Lys(Me,Boc)-OH ea boleng bo holimo, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) 2-OH.HCl(asymmetrical), F li ne li sebelisoa moc-Arg(me)2-OH.HCl(symmetrical) le lisebelisoa tse ling tse tala li entsoe ke FMOC solid-phase synthesis process ho fumana Lys le Arg methylated peptides, le lihlahisoa. li ile tsa hloekisoa ke HPLC.Sehlahisoa se phethiloeng se fanoe ka spectra ea boima bo loketseng, li-chromatogram tsa HPLC le COA.
Nako ea poso: Nov-24-2023