Moralo oa moralo le tharollo ea ketane ea peptide ea polypeptide

I. Kakaretso
Li-peptide ke li-macromolecule tse khethehileng hoo tatellano ea tsona e sa tloaelehang likarolong tsa tsona tsa lik'hemik'hale le tsa 'mele.Li-peptide tse ling li thata ho li kopanya, ha tse ling li batla li le bonolo ho li kopanya empa ho thata ho li hloekisa.Bothata bo sebetsang ke hore li-peptide tse ngata li qhibiliha hanyenyane litharollong tse nang le metsi, kahoo tlhoekisong ea rona, karolo e ts'oanang ea peptide ea hydrophobic e tlameha ho qhibiliha ka li-solvents tse se nang metsi, ka hona, li-solvents tsena kapa li-buffers li ka 'na tsa se ke tsa lumellana haholo le tšebeliso. ea mekhoa ea liteko tsa baeloji, e le hore litsebi li haneloe ka thata ho sebelisa peptide molemong oa tsona, e le hore tse latelang ke likarolo tse 'maloa tsa moralo oa li-peptide bakeng sa bafuputsi.

Moralo oa moralo le tharollo ea ketane ea peptide ea polypeptide
Ea bobeli, khetho e nepahetseng ea li-peptide tse thata tsa maiketsetso
1. Kakaretso ea bolelele ba tatellano e laoloang fatše
Li-peptide tsa masala a ka tlase ho 15 li fumaneha habonolo hobane boholo ba peptide boa eketseha 'me bohloeki ba sehlahisoa se tala boa fokotseha.Ha bolelele bohle ba ketane ea peptide bo ntse bo eketseha ho feta masala a 20, palo e nepahetseng ea sehlahisoa ke taba ea bohlokoa.Litekong tse ngata, ho bonolo ho fumana litlamorao tse sa lebelloang ka ho theola palo e setseng ka tlase ho 20.
2. Fokotsa palo ea masala a hydrophobic
Li-peptide tse nang le masala a mangata a hydrophobic, haholo-holo sebakeng sa masala a 7-12 ho tloha ho C-terminus, hangata a baka mathata a maiketsetso.Sena se nkuoa e le motsoako o sa lekaneng hantle hobane letlapa la B le fumanoa ka har'a motsoako."Maemong a joalo, ho ka ba molemo ho fetolela masala a fetang a mabeli a matle le a mabe, kapa ho kenya Gly kapa Pro ka har'a peptide ho notlolla sebopeho sa peptide."
3. Ho fokotseha ha masala a "thata".
"Ho na le masala a 'maloa a Cys, Met, Arg, le Try ao hangata a sa kopanngoeng habonolo."Ser hangata e tla sebelisoa e le mokhoa o mong oa nonoxidative ho Cys.
Moralo oa moralo le tharollo ea ketane ea peptide ea polypeptide

Ntlha ea boraro, ntlafatsa khetho e nepahetseng ea ho qhibiliha ka metsing
1. Fetola sebaka sa N kapa sa C
Mabapi le li-peptide tse nang le acidic (ke hore, li qosoa hampe ka pH 7), acetylation (N-terminus acetylation, C terminus e lula e boloka sehlopha sa mahala sa carboxyl) e khothaletsoa ka ho khetheha ho eketsa tefiso e mpe.Leha ho le joalo, bakeng sa li-peptide tsa mantlha (ke hore, tse qosoang ka pH 7), amination (sehlopha sa mahala sa amino ho N-terminus le amination ho C-terminus) e khothalletsoa ka ho khetheha ho eketsa tefiso e ntle.

2. Haholo khutsufatsa kapa lelefatsa tatelano

Tse ling tsa tatellano li na le palo e kholo ea li-amino acid tsa hydrophobic, tse kang Trp, Phe, Val, Ile, Leu, Met, Tyr le Ala, joalo-joalo Ha masala ana a hydrophobic a feta 50%, hangata ha ho bonolo ho qhaqha.Ho ka ba molemo ho lelefatsa tatellano ho eketsa lipalo tse ntle le tse mpe tsa peptide.Khetho ea bobeli ke ho fokotsa boholo ba ketane ea peptide ho eketsa lipalo tse ntle le tse mpe ka ho fokotsa masala a hydrophobic.Ha mahlakore a matle le a mabe a ketane ea peptide a le matla, ho na le monyetla o moholo oa ho arabela ka metsi.
3. Kenya masala a qhibilihang ka metsing
Bakeng sa liketane tse ling tsa peptide, motsoako oa li-amino acid tse ntle le tse mpe li ka ntlafatsa ho qhibiliha ha metsi.Khamphani ea rona e khothaletsa N-terminus kapa C-terminus ea acidic peptides hore e kopanngoe le Glu-Glu.N kapa C terminus ea peptide ea mantlha e ile ea fanoa 'me joale Lys-Ls.Haeba sehlopha se qosoang se ke ke sa behoa, Ser-Gly-Ser e ka boela ea behoa sebakeng sa N kapa C terminus.Leha ho le joalo, mokhoa ona ha o sebetse ha mahlakore a ketane ea peptide a ke ke a fetoloa.