Tlhahlobo ea mekhoa ea ho kopanya e kang peptide

Theknoloji ea ho kopanya e kang peptide

Patlisiso le nts'etsopele ea lithethefatsi tsa peptide e ntse e hola ka potlako meriana.Leha ho le joalo, nts'etsopele ea lithethefatsi tsa peptide e lekantsoe ke litšobotsi tsa bona.Ka mohlala, ka lebaka la kutloisiso e khethehileng ea enzymatic hydrolysis, botsitso bo fokotsehile, 'me ho fetoha ha steric conformation ho fella ka ho khetheha ho fokolang, ho fokotseha ha hydrophobicity, le ho hloka tsamaiso e khethehileng ea lipalangoang.Bakeng sa ho hlola li-peptide tsena, litharollo tse ngata tse reriloeng le ts'ebeliso e atlehileng ea mofuta o le mong oa peptide ke e 'ngoe ea tsona.

Mofuta oa peptide (lebitso la Senyesemane: Peptoid) kapa Poly - N - sebakeng sa glycine (lebitso la Senyesemane: Poly real - N - substitutedglycine), ke metsoako ea quasi peptide ea peptide ketane e kholo.Ketane ea mahlakoreng ea alpha carbon e fetisetsa ketane e kholo ea nitrogen sebakeng sa ketane ea mahlakoreng.Ho polypeptide ea mantlha, sehlopha sa R ​​sa ketane ea lehlakore ea amino acid e emetse li-amino acid tse 20 tse fapaneng, empa sehlopha sa R ​​se na le likhetho tse ngata ho peptoid.Ho peptide, peptide ho ketane e kholo ea li-amino acid ka alpha carbon nitrogen sebakeng sa phetiso ea ketane e ka thoko ho ketane e kholo.Ho bohlokoa ho bolela hore li-peptide ka kakaretso ha li hlahise meaho e tšoanang ea maemo a holimo joalo ka meaho ea bobeli ea li-peptide le liprotheine ka lebaka la khaello ea hydrogen mokokotlong oa naetrojene.Sepheo sa pele sa Peptide ke ho hlahisa peptide e tsitsitseng le ea protease ea lithethefatsi tse nyenyane tsa molek'hule.

Tlhahlobo ea mekhoa ea ho kopanya e kang peptide

Mokhoa oa peptide synthesis o ile oa hlahisoa

Mokhoa o tsebahalang ka kakaretso o kang oa peptide ke mokhoa oa subsingle synthesis o qapiloeng ke RonZuckermann, e 'ngoe le e' ngoe e arotsoe ka mehato e 'meli: acylation le displacement.Ho acylation, mohato oa pele ke ho kenya haloacetic acid hore e sebetsane le li-amine tse setseng qetellong ea mohato o fetileng, hangata diisopropyl carbonized diimine.bromoacetic acid e ne e sebelisoa ke diisopropylcarbodiimide."Liphetohong tsa phetoho (bimolecular nucleophilic substitution reactions), amine, hangata e le ea mantlha, e hlasela halogen e 'ngoe ho etsa glycine e nkeloang sebaka ke N."Tsela ea subunitary synthetic e sebelisa li-amine tsa mantlha tse fumanehang habonolo ho hlahisa li-peptide, ka hona e nolofalletsa motsoako oa lik'hemik'hale oa li-peptide.

Katoloso e tiileng ea sehlopha sa li-peptide synthesis e na le boiphihlelo bo bongata, e ka u fa mefuta e fapaneng ea ts'ebeletso ea peptide synthesis.

Tlhahlobo ea mekhoa ea ho kopanya e kang peptide

Molemo oa peptide e joalo

E tsitsitse haholoanyane: li-peptoids li tsitsitse ho feta li-peptide.

Khetho e eketsehileng: Li-Peptoids li loketse hantle bakeng sa lithuto tse kopantsoeng tsa ho sibolla lithethefatsi hobane mefuta e mengata e fapaneng ea li-polypeptide e ka fumanoang ka ho fetola sehlopha sa amino sa mokokotlo.

E sebetsa hantle haholoanyane: Bongata ba meaho ea peptoid bo ka etsa hore peptoid e be khetho e ntle bakeng sa mokhoa oa ho skena ho fumana kapele meaho e itseng e tlamahanyang liprotheine.

Monyetla o eketsehileng oa 'maraka: litšobotsi tsa mofuta oa peptide e ke e fetohe mofuta oa nts'etsopele ea lithethefatsi e na le bokhoni bo boholo.