Dipeptide-6/18684-24-7/GT Peptide/Peptide Supplier
Tlhaloso
Dipeptide-6e hlaha e le phofo e tshweu ho isa ho e tshweu mme e qhibiliha metsing.Ho lumellana hantle le letlalo.Ho bonolo ho monya.Molemo o khahlanong le botsofali, phello ea tšireletso ea letlalo.
Litlhaloso
Ponahalo: Phofo e tšoeu ho isa ho e tšoeu
Bohloeki(HPLC):≥98.0%
Tšilafalo e le 'Ngoe:≤2.0%
Litaba tsa Acetate(HPLC): 5.0%～12.0%
Litaba tsa Metsi (Karl Fischer):≤10.0%
Litaba tsa Peptide:≥80.0%
Ho Paka le ho Romela: Mocheso o tlase, ho paka ka vacuum, ho nepahetse ho mg ha ho hlokahala.
Joang Ho Odara?
1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.
2. Odara inthaneteng.Ka kopo, tlatsa foromo ea inthaneteng.
3. Fana ka lebitso la peptide, Nomoro ea CAS kapa tatelano, bohloeki le phetoho ha ho hlokahala, bongata, joalo-joalo re tla fana ka khotheishene nakong ea lihora tse peli.
4. Khokahano ea taelo ka konteraka ea thekiso e saenneng ka nepo le NDA(tumellano ea ho se senole) kapa tumellano ea lekunutu.
5. Re tla tsoela pele ho ntlafatsa tsoelo-pele ea taelo ka nako.
6. Peptide e fana ka DHL, Fedex kapa ba bang, le HPLC, MS, COA e tla fanoa hammoho le thepa.
7. Leano la puseletso le tla lateloa haeba ho na le phapang ea boleng kapa tšebeletso ea rona.
8. Ts'ebeletso ea kamora thekiso: Haeba bareki ba rona ba na le lipotso mabapi le peptide ea rona nakong ea liteko, ka kopo ikutloe u lokolohile ho ikopanya le rona 'me re tla e araba ka nako e khutšoane.
Lihlahisoa tsohle tsa k'hamphani li sebelisoa feela molemong oa lipatlisiso tsa mahlale, eona'e thibetsoe ho sebelisoa ka kotloloho ke motho ofe kapa ofe 'meleng oa motho.
LBH
Mefokolo ea bolelele ba li-peptide tsa maiketsetso ke efe?
Polypeptide e entsoeng ke k'hamphani ea rona e bolelele ba 6 ~ 50 amino acid.Mekhoa e tloaelehileng ea "solid-phase synthesis" hangata e hlahisa li-peptide tsa 6 ho isa ho 50 amino acid.
Mokhoa oa ho hlalosa tlhoro ea P+Na le P+K ho MALDI(MS)?
Litlhōrō tsa Na le K hangata li bonoa MALDI, 'me sodium le potasiamo li tsoa metsing a qhibilihisang.Esita le metsi a silafalitsoeng le a hloekisitsoeng a ka ba le li-ion tsa sodium le potasiamo tse ke keng tsa tlosoa ka ho feletseng.Li boetse li kopanya le ho tlama ho sehlopha sa mahala sa carboxyl sa peptide nakong ea spectrometry ea boima.Hobane ha ho na mokhoa oa ho hloekisa ho tlosa li-ion tsa sodium le potasiamo metsing, ka linako tse ling ho ke ke ha qojoa hore litlhōrō tsa sodium le potasiamo li hlahe 'mapeng oa MALDI MS.
Ka kopo hlalosa leano la hau la tlhoekiso
Li-peptide tse entsoeng ke k'hamphani ea rona li ile tsa hloekisoa ke HPLC e lokiselitsoeng morao-rao e nang le TFA le pH 2 e kenyellelitsoeng mekhahlelong ka bobeli ea mehala.Mokhahlelo oa A ke 0.1% TFA metsing a ultra-pure, 'me mohato oa B ke 0.1% TFA ho ACN, pH 2. Mohlala o ka qhibiliha ka ho toba karolong ea A, kapa oa qhibiliha ka A karolo e nyenyane ea B le ho hlapolloa ka A phase.Ka linako tse ling ho ka 'na ha hlokahala ho qhaqha li-peptide tsa hydrophobic ka li-solvents tse matla tse kang formic acid kapa acetic acid, ho itšetlehile ka tatellano ea peptide.Ho pH 6.8, hangata ho thata ho qhibiliha le ho hloekisa peptide, kahoo hangata re qhala peptide pele ebe re sebelisa mekhahlelo e 'meli e tsamaeang bakeng sa khanya ea gradient.Tharollo ea "buffer" ho pH 6.8 e ne e le 10 mM ammonium acetate metsing a hloekileng haholo (mohala oa A), ACN e hloekileng (mohato oa B).Likarolo tse fapaneng li ile tsa bokelloa 'me tsa khetholloa ke MADLI-TOF MS.Purity e ile ea hlahlojoa ke HPLC ea morao-rao.Joale, li-peptide tse shebiloeng li ne li le lyophilized, 'me li-peptide tse nang le lyophilized li kopantsoe ka linkho tse nyane.
U beha leihlo lihlahisoa tsa hau joang?
Li-peptide tsohle tsa maiketsetso li ile tsa hlahlojoa ke HPLC le MS, ntle le MALDIMS.Kaha li-peptide li na le ionized ka tatellano ea tsona ka li-spectrometer tse fapaneng tsa boima, mekhoa ea HPLC-MS e ka sebelisoa ho sekaseka litlhoro tseo tsa ionization ho HPLC.Thepa ea rona ea theknoloji [MALDI-MS, HPLC-(ESI)MS (ion trap and tetrode array)] e fana ka tiisetso e tšepahalang bakeng sa tlhahlobo.
Na ho qhibiliha ha peptide ho amana le boleng ba peptide?
Li-peptide tsa maiketsetso ha li qhalane hantle, li-peptide li na le bothata, na ha ho joalo?
K: Ho thata ho bolela esale pele hore na peptide e qhibiliha hakae le hore na solvent e nepahetseng ke eng.Ha se 'nete hore ho na le bothata ba peptide synthesis haeba ho le thata ho qhaqha.
U boloka li-peptide joang ka tharollong?
Haeba o tlameha ho boloka li-peptide tsa hau ka har'a metsi, sebelisa buffer e hloekisitsoeng ho PH 5-6 'me u boloke ho -20.℃ho lelefatsa bophelo ba li-peptide tsa hau ka tharollo.
Li-peptide li sebetsa nako e kae ka tharollo?
Ho molemo hore u se ke ua boloka li-peptide tse setseng ka tharollo.Bophelo ba sethala sa polypeptides ka tharollo bo fokotsehile haholo, haholo-holo ba nang le cysteine, methionine, tryptophan, asparagic acid, glutamic acid, le N-terminal glutamic acid ka tatellano.Ka kakaretso, tlosa palo e hlokahalang ea tšebeliso, karolo e setseng ea leqhoa e omisitsoeng bakeng sa polokelo ea nako e telele.
